fitc  (Bio-Rad)

Journal: International Journal of Molecular Sciences

Article Title: A New Method of Canine CD4 + T Lymphocyte Differentiation Towards the Th17 Phenotype with Analysis of Properties and Mitochondrial Activity

doi: 10.3390/ijms26104946

Figure Lengend Snippet: Canine CD4 + T lymphocytes were effectively activated using magnetic EpoxyBeads. Canine CD4 + T cells were isolated with the pluriBead Cell Separation Kit and analyzed by multicolor flow cytometry (FASC Aria II, Becton Dickinson). ( A ) Gating strategy for flow cytometry analysis. T lymphocytes were gated based on FSC and SSC. Only singlets and viable cells (v450 negative) were gated for further analysis. Among the analyzed cells, the vast majority consisted of CD5 + T cells. ( B ) Within T lymphocytes group, two subpopulations were distinguished based on CD4 and CD8α surface expression. ( C ) Isolation of CD4 + T cells with the pluriBead Cell Separation Kit allowed us to obtain high population purity (above 90%). ( D ) Bar graph showing mean percentage of activated canine CD4 + T cells 24 h post-stimulation with EpoxyBeads and ConA. Data are shown as the mean results of three separate isolations ( n = 3), and error bars indicate SEM. Statistical analysis was performed by One-way analysis of variance (ANOVA) with Tukey’s Multiple Comparison Test (** p < 0.01, *** p < 0.001). ( E ) Representative cytograms of CD25 expression in CD4 + T lymphocytes after stimulation with different EpoxyBeads to CD4 + T cells ratios (FACS Aria II, Becton Dickinson).

Article Snippet: The following antibodies were used to analyze the expression level of surface molecules and activation markers: anti-canine CD4-APC (clone YKIX302.9), anti-canine CD8a-v450 (clone YCATE55.9), anti-canine CD5-PerCP-eFluor710 (clone YKIX322.3), and anti-canine CD25-FITC (clone P4A10) (all from eBioscience™, Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Isolation, Flow Cytometry, Expressing, Comparison

Journal: PLOS One

Article Title: Immune mechanisms affected by cyclooxygenase inhibition combined with antiviral treatment in calves infected with bovine respiratory syncytial virus

doi: 10.1371/journal.pone.0321642

Figure Lengend Snippet: Antibodies used for flow cytometry.

Article Snippet: Mouse anti-bovine CD25 , IL-A111 , FITC , Bio-Rad.

Techniques: Cytometry